Reporter
UAS-EGFP

Part:BBa_K758006:Design

Designed by: Takaaki Takeda   Group: iGEM12_KIT-Kyoto   (2012-09-18)


UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1130
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1130
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1130
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is made by using BBa_K758003. This part of EGFP is amplified by PCR pEGFP-C2.


Source

UAS sequence, EGFP, and SV40 polyadenylation signal

References